Journal: Frontiers in Microbiology
Article Title: Plasmid-borne transcriptional regulator RamAp modulates Salmonella genes for environmental and host adaptation
doi: 10.3389/fmicb.2026.1842592
Figure Lengend Snippet: RamAp enhances superoxide dismutase (SOD) activity in Salmonella LT2. SOD activity was measured in crude lysates of S. enterica serovar Typhimurium LT2 carrying either pBR322 or pBR322-IS Ecp1* - ramAp using a commercial assay kit. Activity values were normalized to total protein concentration. Each data point represents an independent biological replicate ( n = 3); horizontal lines indicate the mean and standard deviation. A significant increase in SOD activity was observed in the ramAp -expressing strain.
Article Snippet: Crude lysates were prepared and analyzed using the CheKineTM Micro Superoxide dismutase (SOD) Activity Assay Kit (Abbkine, Atlanta, GA, USA), following the manufacturer’s instructions.
Techniques: Activity Assay, Protein Concentration, Standard Deviation, Expressing